Neshek, who battled a sore flexor tendon in the middle finger of his pitching hand, made an appearance for Class A Fort Myers on Friday. He threw one inning, allowing three earned runs on three hits and two walks.
"He's just like everyone else in the Minor Leagues now," said Twins manager Ron Gardenhire. "He's got to pitch his way back up. When there's a need, he'll get an opportunity -- if he's the one throwing the ball good."
The 29-year-old missed all of 2009 after undergoing Tommy John surgery, but broke this year's Spring Training with the big league club.
Neshek made six appearances for the Twins in April before being placed on the 15-day disabled list May 2. Over 4 1/3 innings with Minnesota this year, he allowed two earned runs on two hits and two walks while striking out four.