Plouffe left Sunday's game against the Pirates in the seventh inning after experiencing tightness in his hamstring upon rounding first base after hitting an RBI single.
"I felt a grab in my lower hamstring while rounding first base," Plouffe said. "I knew what it was, because I had it happen in Boston last year. But this one is a lot less severe. So I'm happy."
Plouffe strained his left hamstring on May 8 last season before returning on May 13. The Twins have an off-day Tuesday, but Plouffe said he's not sure if he'll be ready to go by Wednesday.
"I hope Wednesday, but I'd definitely rather take it easy and make sure it's healed up," said Plouffe, who estimated it might take a week before his hamstring is fully healed.
Plouffe had been hitting well over his previous four games, going 6-for-12 with a double and two RBIs to raise his batting average to .276 this spring.
"We have a bunch of good guys to work with here, and it was coming around," Plouffe said. "The last couple games had been going good for me. I think it'll still be there when I get back."