Plouffe, who went through workouts without any issues on Monday and Tuesday, served as designated hitter against Triple-A Norfolk at Minnesota's Spring Training complex.
He was observed by general manager Terry Ryan and manager Ron Gardenhire before the Twins' Grapefruit League game against the Phillies at Hammond Stadium.
"All I wanted to do was watch him run the bases, and all he did was trot," Gardenhire joked. "It was a waste of my time to go see him hit the ball over the fence and trot around the bases when we wanted to see if his hamstring is OK."
Additionally, Ryan said that Sean Burroughs had an X-ray taken on his left forearm after being hit by a pitch there on Monday, but that it came back negative. Burroughs remains day to day.
Outfielder Josh Willingham also made his return to the lineup on Wednesday after missing two games with a sore right ankle.