But Twins manager Ron Gardenhire said it's such a minor injury that he didn't even know which hamstring was bothering Span.
"Span had a little bit of a sore hammy after the game," Gardenhire said. "It's no big deal. He's available, but we didn't want to push that. He went and got treatment and everything."
With Span out, Darin Mastroianni made his first start in center field and batted leadoff. Catcher Joe Mauer also batted second for the first time this season, while rookie shortstop Brian Dozier was moved into the fifth spot in the order.
"I thought about a lot of different lineups and a lot of different things," Gardenhire said. "We're just mixing it up a little bit -- see what happens."