Mauer, who sprained his right thumb while swinging the bat on Sunday, missed four games with his injury. But he was back at catcher and batting third on Saturday.
Span, meanwhile, was out of the starting lineup on Friday with flu-like symptoms. He served as a pinch-runner late in the game and felt much better on Saturday. He started in center and was back in his usual leadoff spot in the order.
"They're in; they're good," Twins manager Ron Gardenhire said. "Denard came in and said he was feeling good today. And Joe is ready to go. He's ready to catch. The whole package."