Plouffe injured his thumb while getting jammed on a pitch from Royals right-hander Luke Hochevar on Friday.
But Twins manager Ron Gardenhire expects Plouffe to be back in the lineup against the White Sox on Monday.
"He came in today and had a lot of improvement," Gardenhire said before the rubber game against the Royals. "But in my experience in getting jammed as a player, which was plenty and often, one day doesn't let it heal. You need a couple days to regroup. If you go right back at it, then it doesn't go away for a long time. So I'm giving him an extra day, but I'm guessing he'll back in there tomorrow."
With Plouffe out of the lineup, Jamey Carroll made his second straight start at third base.