He had precautionary X-rays taken on his shoulder after the play but they came back negative. Twins manager Ron Gardenhire said Span is day to day.
"He's pretty sore in his collarbone and shoulder area," Gardenhire said.
The injury occurred when Span tried to catch a sinking liner from Jeff Keppinger that ended up as a double. Span slid to his knees before awkwardly falling on his shoulder trying to make the catch.
Span initially stayed in the game after being looked at by a trainer and Gardenhire in center field, but was later removed after the inning.
Ben Revere shifted from right field to center field, while Darin Mastroianni took over in right field.