Span was originally slated to start in center field and lead off for Minnesota, but he was later removed due to shoulder soreness.
"He's day to day. He's got a sore collarbone, is what it is," general manager Terry Ryan said. "He's available if we need him, but I was going to give him a day. I don't think he did anything to it. It's one of those things that's sore. I don't think anybody's all that concerned about it, but he's sore today."
Ben Revere shifted over from right to Span's usual spot in center and hit in the leadoff spot. Darin Mastroianni was slotted in right field and hit second.
Span missed a string of nine straight games from Aug. 13-22 after injuring his collarbone while trying to make a sliding catch on Aug. 12. He missed another game on Aug. 24 after returning a day earlier, but he had played three straight games from Saturday-Monday.
The 28-year-old is hitting .286 this season.