In a corresponding move, infielder Pedro Florimon was recalled from Triple-A Rochester.
The team originally called the injury to Plouffe an oblique strain.
"It's kind of in the same family, but it's not as severe as an oblique strain," assistant general manager Rob Antony said. "We're hoping it won't take quite as long."
The injuries to Plouffe and Eduardo Nunez, who aggravated his right hamstring after entering as Plouffe's replacement on Saturday, further compound the Twins' injury woes this season.
"It does make it a little more interesting," Twins manager Ron Gardenhire said of playing shorthanded. "You know you're going to have to every once in a while. Continuous playing short is not good. We want to give ourselves every chance and every option."
Florimon was scheduled to arrive to Comerica Park around game time Sunday.
Matt Slovin is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.