After the review, the call was overturned and Nunez was ruled safe at first base. But after the overturned call, Nunez was promptly picked off at first base by Royals catcher Salvador Perez.
Gardenhire has now issued 24 challenges on the season, and has had 12 calls overturned. He lost a challenge in Tuesday's 2-1 win over the Royals and was ejected for arguing with umpire Ted Barrett.
Rhett Bollinger is a reporter for MLB.com. Read his blog, Bollinger Beat, and follow him on Twitter @RhettBollinger. This story was not subject to the approval of Major League Baseball or its clubs.